Exploring the regulatory mechanism of Nao Tai Fang on vascular Dementia's biological network based on cheminformatics and transcriptomics strategy

Nao Tai Fang (NTF) is modified from Buyang Huanwu Decoction. Modern pharmacological research showed that NTF has a good anti-cerebral ischemic effect and can improve the learning memory ability of cerebrovascular disease. The purpose this study to explore regulation mechanism on vascular dementia (VD)'s biological network based chemoinformatics transcriptomics strategies. First, bilateral common carotid artery ligation method was used create rat VD model. After intervention for 30 days, treatment evaluated by HE staining water maze experiment. Then, Agilent mRNA expression profiling chip obtain data hippocampal tissues model rats before after intervention, microarray analysis screen genes with significant differential expression. BATMAN database utilized potential targets Genecards OMIM were collect genes. cytoscape construct analyze networks. animal experiments VD. A total 180 up-regulated proteins 289 down-regulated identified. top 20 up- differentially expressed gene's protein-protein interaction (PPI) network. 677 550 obtained NTF-VD PPI cheminformatics regulate lot processes signaling pathways (such as inflammation modules, vasodilation contraction hypoxia angiogenesis, coagulation, neurovascular unit Neuroactive ligand-receptor interaction, Calcium pathway, Serotonergic synapse). may play role in through targets, discovered study.